Bacterial population genomics and infectious disease diagnostics sandeep j. The microarray data provided in gene profiling and gene expression profiling are quantitative 6789 19. Diagnosis and monitoring of disease are key to clinical management. Integrated amplification microarrays for infectious disease. In contrast with other proteomic techniques such as 2d gel electrophoresis and mass spectrometry, the arrayed probes. Diagnosis and treatment of infectious disease list of high. Disease applications applied microarrays microarray. Panel infectious disease testing to aid in the diagnosis of infectious. Taking connected mobilehealth diagnostics of infectious. Application of a cell microarray chip system for accurate. Therefore, the use of dna microarray is a prompt approach to diagnose a wide range of noninfectious diseases such as cancers, leukemia, lymphoma, melanoma, diabetes, arthritis, systemic lupus erythematosis sle, rheumatoid arthritis ra, and crohns disease via biomarker genes6,8. Applications of dna microarray in disease diagnostics.
Different types of laboratory tests are useful for identification of microorganism are stained and examining, cultured, tested for antibodies to organism and testing for genetic material. Dna microarray technology offers a new way to clinical diagnostics involving biomarker discovery and disease diagnosis. Microarray technology has been utilized in the identification of various infectious disease pathogens such as avian influenza. Microarray technology is a powerful tool to investigate whole genome expression profiles to study the crosstalk between pathogens and associated hosts that cause illness. Jan 21, 20 microarray technology in microarray diagnosis, the known dna large oligonucleotides or complementary dna are immobilized on a glass slide, and the unknown dna labeled probe is in the liquid phase. The role of microarray technology in pharmaceutical. Using dna microarrays to study hostmicrobe interactions. Until recently, different types of cancer have been classified on the basis of the organs in which the tumors develop. Dna microarrays for the diagnosis of infectious diseases. Rapid differential diagnosis of infectious diseases is increasingly important as novel pathogens emerge in new contexts and treatment strategies are beginning to be tailored to specific infectious agents. Complete genomic sequences of microbial pathogens and hosts offer sophisticated new strategies for studying hostpathogen interactions. We developed a new oligonucleotide microarray comprising 16 identical subarrays for simultaneous rapid detection of avian viruses.
Advanced diagnostics for infectious disease improving clinical outcomes through novel technology and molecular testing august 2627, 2020. Proposed proprietary laboratory analyses panel meeting agenda. This study is the first clinical application of the solgel protein microarray detection system and provides an important new practical screening technology to advance the current state of infectious disease diagnosis. Microarrays have potential to provide a platform for highly multiplexed differential diagnosis of infectious diseases 4,5. Read1,2 1department of medicine, division of infectious diseases, emory university school of medicine, 615 michael street, atlanta, ga 30322, usa 2department of human genetics, emory university school of medicine, 615 michael street, atlanta, ga 30322, usa. May 11, 2011 genomics can be used for early diagnosis, especially in the field of infectious diseases. Development of oligonucleotide microarray for accurate and. Protein microarrays are increasingly used in infectious disease research to identify new biomarkers that are involved in the disease process or that are targets of immune responses 8. Pcr has revolutionized the field of infectious disease diagnosis. Experience with rapid microarraybased diagnostic technology. Remember the gentleman who flew to italy on his honeymoon but who had tuberculosis. It led to an international concern that he might have infected others and that his.
The development of new dna microarrays systems for fungal detection is relevant for clinical diagnostics of fungal infection dis eases 57. A microarray is socalled because it consists of 20,000 or more different known dnas, each dna being spotted onto glass slides, to form the. Pdf we argue that the mostpromising area of clinical application of microarrays in the foreseeable future is the diagnostics and monitoring of. Infectious disease society of america, the american academy for the study of liver diseases, and the pediatric infectious disease society.
Dept of pediatrics, division of infectious diseases and center for vaccines and immunity, the research institute at nationwide childrens hospital and the ohio state university college of medicine. The diagnosis of virus types based on microarray technology can improve the quality and shorten the analysis duration in molecular diagnosis of infectious diseases. One approach to multiplex detection, characterization, and monitoring of infectious diseases is microarray analysis. Since the beginning of the century, several microarray systems have been developed that have the potential for simultaneous. Integrated amplification microarrays for infectious. Thus, dna microarrays will play a central role in clinical diagnostics and will become a gold standard method for disease diagnosis. Infectious disease molecular diagnostics market size. The performance of dnamicroarraybased diagnosis is continuously improving by the integration of other tools. In the current diagnostics market, molecular diagnostics for infectious disease testing offer one of the brightest areas for growth and innovation. Furthermore, the quantitative and qualitative aspect of microarrays may eventually be exploited to screen for molecular markers of head and neck cancer. Panmicrobial oligonucleotide array for diagnosis of.
Diagnosis of pathogens using dna microarray bentham science. An easy operating pathogen microarray eopm platform for. Furthermore, some discrepancies in detection of mixed infections were. Jul 22, 2016 accurate, sensitive, rapid and easy operative diagnosis is necessary to prevent the spread of malaria. Mm22 specifically addresses infectious disease detection, identification, and genotyping, as well as drug. We view dna microarrays as one in a suite of tools to be used for infectious disease diagnosis and surveillance. The control of infectious diseases represents a unique challenge because infections can be transmitted to others and thus. A cell microarray chip system including a push column for the recovery of erythrocytes and a.
The objective of the study was to develop an oligonucleotide microarray for rapid diagnosis of avian di seases that would be used in the course of mass analysis for routine epidemiological surveillance owing to its a bility to test one specimen for several infections. Basic concepts of microarrays and potential applications in clinical. Microarrays have been used in several comparative genome hybridization studies of pathogens. Bacterial population genomics and infectious disease. Approved guideline this document provides guidance for the laboratory development and use of qualitative nucleic acid microarray methods for the diagnosis and monitoring of infectious diseases. However, the progression of the molecular diagnostic revolution currently relies on the ability to efficiently and accurately offer multiplex detection and characterization for a variety of infectious disease pathogens.
Microarrayinatube for detection of multiple viruses. One of the earliest investigations into the biology of pneumococci led to the landmark 1944 discovery that genes are made of dna and not protein. The further advancement of molecular infectious disease diagnostics is. The availability of a large number of microbial genome sequences generated by conventional sanger sequencing and next generation. Dna microarray and its applications in disease diagnosis. Examples of the disease areas covered by arrays produced by ami include. Dna microarray for detection of gastrointestinal viruses journal of.
Host transcriptomics for diagnosis of infectious diseases. The design and construction of a dna microarray for any given microbial genome are straightforward. Infectious diseases detection by microarray scientific research. Microarray technology has been utilized in the identification of various infectious disease pathogens such as avian influenza h5n1, fmd, viral hemorrhagic fever marburg. Nov 15, 2019 the development of protein chip or microarray technology has provided a highly sensitive, highthroughput method for disease diagnosis by facilitating detectionin small amounts of patient samplesof antibodies to antigens from infectious viruses or bacteria. High diagnostic accuracy of antigen microarray for sensitive detection of hepatitis c virus infection jungah kwon. Microarray detection is a good method, but requires complex procedures for multiple virus detection. The importance of dna microarrays in research for disease diagnosis, understanding drug interactions and determining drug candidates is expected to keep the market growing substantially.
Microarray technology will accelerate our understanding of the complex genetic processes underlying the interaction between microorganisms and the host, with consequent improvements in the diagnosis, treatment, and prevention of infectious diseases. Although the use of microarrays to generate gene expression data has become routine. Abstract technological developments such as microarray. We developed a novel pcr assay, the microarray in atube system, which integrates multiple pcr processes and dna microarrays for. Initially, a pepperchip infectious disease epitope microarray was incubated with secondary and control antibodies to investigate background interactions with infectious disease epitopes. Advances in technology have opened opportunities for new targets, and rapid molecular tests have greatly reduced the time it takes to diagnose infectious disease. Oct 26, 2018 microarrays may eventually help in the understanding of the disease and ultimately lead to improvements in diagnosis, treatment, and outcome. The detection of multiple viruses is important for pathogenic diagnosis and disease control. Detection of respiratory viruses and subtype identification. Diagnosis of pathogens using dna microarray request pdf. Doctors suspect an infection based on the persons symptoms, physical examination results, and risk factors.
The dna microarray and infectious diseases combination, gives 721 references may 2012. Microarray technology in microarray diagnosis, the known dna large oligonucleotides or complementary dna are immobilized on a glass slide, and the unknown dna labeled probe is in the liquid phase. Application note, microarray applications in infectious. The major advantages to these systems are the reproducibility of the. The uses of dna microarrays in infectious diseases. Experience with rapid microarray based diagnostic technology and antimicrobial stewardship for patients with grampositive bacteremia volume 37 issue 11 elizabeth a. A chip targeting the variable regions of the gyrb and pare of the bacterial genes detects corynebacterium diphteriae, fusobacterium necrophorum, haemophilus influenza, legionella pneumophila, moraxella catarrhalis, mycoplasma pneumoniae. Infectious diseases are caused by microorganisms, such as bacteria, viruses, fungi, and parasites. Although microarray technology is still in its infancy, further investigation may prove helpful in the diagnosis, prognosis, and management of head and neck cancer.
Three dna microarrays were designed for the diagnosis of respiratory infections. Using genomics in cancer, and for early diagnosis in. New oligonucleotide microarray for rapid diagnosis of avian. Dna microarrays for virus detection in cases of central nervous system infection. Clinical application of microarray technologies for the detection of mycobacteria, bacillus anthracis, hiv, hepatitis and influenza viruses, and other. Accurate, sensitive, rapid and easy operative diagnosis is necessary to prevent the spread of malaria. In addition to detection and identification of pathogens, microarrays are ideal for characterizing genetic differences between.
New oligonucleotide microarray for rapid diagnosis of. Moreover, it can satisfy the needs of simultaneous detection of multiple viruses and screening large numbers of pathology samples 32, 33. We focused the development and validation of our technology on this infectious disease because it is the leading cause of death in developing countries causing around 4 million deaths annually 2,24. Diagnostic applications of microarray include physiologic and biochemical diseases, infectious diseases, and genetic malfunctions and dysregulations in which the produced data are qualitative.
The number of potential features per microarray far exceeds those of any other known technology. Dna microarrays exploit primary sequence data to measure transcript levels and detect sequence polymorphisms, for every gene, simultaneously. While most infectious disease microarray research has focused on. Diagnosis and treatment of infectious disease list of. Since the draft sequence of the human genome was published in 2001, the cancer genome anatomy project index of tumor genes has classified more than 40,000 genes directly or indirectly involved in one or more cancers. An understanding of the host immune system is vital to a. Infectious disease testing to aid in the diagnosis of respiratory infections, fully automated multiplexed nucleic acid test dna and rna for detection of 20 respiratory. Diagnosis by restriction fragment length polymorphisms and related dnabased approaches 3. Microarray summary lots of variations glass, nylon long, short dna molecules fab via photolithography, ink jet, robot radioactive vs fluorescent readout relative vs absolute intensity leads to diverse sensitivity, bias, noise, etc. Experience with rapid microarraybased diagnostic technology and antimicrobial stewardship for patients with grampositive bacteremia volume 37 issue 11 elizabeth a. Therefore, a rapid and costeffective pathogen screening method with broad coverage is required for prevention and control of infectious diseases. New microarray assay for ebola diagnosis by melissa j.
Genomics can be used for early diagnosis, especially in the field of infectious diseases. Moreover, it can satisfy the needs of simultaneous detection of multiple viruses and. Basic concepts of microarrays and potential applications. The use of complementary dna cdna microarray technology to explore gene expression on a global level is rapidly evolving. The performance of dna microarray based diagnosis is continuously improving by the integration of other tools. Gastroenteritis stands among the five principal causes of mortality by disease and morbidity at all ages worldwide. Microarrays for diagnosis and monitoring of infectious.
We developed a novel pcr assay, the microarrayinatube system, which integrates multiple pcr processes and dna microarrays for multiple virus detection. Dna microarray can be used for diagnosis of pathogens by analyzing hybridization patterns between capture probes and nucleic acids isolated from clinical samples. Multiplex pcr, a variant of the test in which more than one target sequence is amplified using more than one pair of primers, has also been developed 17. Innovative dendrischips technology for a syndromic approach. Precision medicine for public health posted on september 24, 2015 by marta gwinn, consultant, mcking consulting corp, office of public health genomics and duncan maccannell ncezid, centers for disease control and prevention.
By characterising both major and minor genomic variations, microarrays can be. It led to an international concern that he might have infected others and that his tb might be of the drug resistant variety. Microarray analysis has the capability to offer robust multiplex detection but has just started to enter the diagnostic. Application note, microarray applications in infectious disease pdf. Microarrays for diagnosis and monitoring of infectious diseases. First, doctors confirm that the person has an infection rather than another type of illness. Proposed proprietary laboratory analyses panel meeting. Basic concepts of microarrays and potential applications in. Microarray technologies in the diagnosis and treatment of. It also presents recommendations for validation and verification, quality control, and. Microarray technologies conclusion and potential for adoption. Infectious diseases emerge frequently in china, partly because of its large and highly mobile population. Dna microarray technology helps researchers learn more about different diseases such as heart diseases, mental illness, infectious disease and especially the study of cancer. Histogram of dna microarray topics published in the pubmed database from 1995 to 2012 may 2012.
Infectious diseases are caused by several microorganisms. To get information on various segments, share your queries with us the global infectious disease molecular diagnostics market is anticipated to grow owing to the increasing prevalence of infectious diseases, rising awareness regarding prevention and early diagnosis of sexually transmitted diseases, and active government support. Early diagnosis of an infectious disease is always desirable to prevent its spread among livestock species and thus reduce the economic losses. High diagnostic accuracy of antigen microarray for. Microarray applications in infectious disease application notes infectious disease application note modern biology and genomic sciences are rooted in infectious disease research. High diagnostic accuracy of antigen microarray for sensitive. It is anticipated that integrated microarray biochemistry will provide an opportunity to significantly reduce the complexity and cost of microarray consumables, equipment, and workflow, which in turn will enable a broader spectrum of users to exploit the intrinsic multiplexing power of microarrays for infectious disease diagnostics. One of the most common applications of microarrays in infectious disease has been in epidemiological investigation.